直接测序法检测结肠癌患者K-ras基因的灵敏度及其临床价值分析

目的 探讨结肠癌患者应用直接测序法测定K-ras 基因的灵敏度及其临床价值。方法 选取我院2016 年2 月~2017 年4 月收治的结肠癌患者80 例，随机分为研究组和对照组两组，研究组患者通过直接测序法、对照组患者通过sanger 测序法检测患者体内的K-ras 基因突变情况，分析K-ras 基因突变率与患者肿瘤大小、肿瘤部位、患者年龄等一般资料之间的相关性。结果 研究组患者测定K-ras 基因突变的灵敏度明显高于对照组（P<0.05），肿瘤直径在5 cm 以上患者的K-ras 基因突变率明显低于直径在5 cm 以下的患者（P<0.05）研究组检出率与患者肿瘤部位及分化程度、患者年龄等存在相关性。结论 应用直接测序法检测结肠癌患者K-ras 基因的突变情况具有较高的灵敏度，直接测序法的检出率与患者的肿瘤大小、肿瘤分化程度以及患者的年龄等存在较强的相关性，对于临床上结肠癌等恶性肿瘤疾病的诊断具有较强的应用价值，值得临床上广泛推荐使用。     

Last Observation Carried Forward: A Crystal Ball?

In spite of widespread criticism and notwithstanding the availability of flexible, broadly valid, and conveniently implemented methods like direct-likelihood and direct-Bayesian analyses, last observation carried forward (LOCF), baseline observation carried forward (BOCF), and complete case analysis remain popular for handling incomplete clinical trial data. To the well-known criticisms toward LOCF and BOCF, namely, that they can be either conservative or liberal depending on the context, that they can violate the intention-to-treat principle, and so on, we add that, with the exception of certain degenerate and unrealistic special cases, these methods are incompatible with the sensible requirement that the mechanism governing dropout be independent of future, unobserved measurements, given covariates, past observed measurements, and the current possibly missing measurement.     

电针双膝眼加温灸治疗膝骨关节炎疗效观察

目的：评价电针加温灸治疗膝骨关节炎（KOA）的临床效果。方法：把100例KOA患者随机分为2组,每组各50例。治疗组采用电针双膝眼、阳陵泉、足三里加温灸治疗,对照组采用电针双膝眼、阳陵泉、足三里加TDP治疗,均每日1次,10次为1个疗程。1个疗程后比较2组疗效。结果：治疗组与对照组总有效率分别为100%和92%,2组比较无显著性差异（P〉0.05）;但2组在膝关节功能改善指数、改善率、治愈率、显效率方面存在显著性差异（P〈0.01）。结论：电针双膝眼加温灸治疗KOA疗效显著,治愈率高,值得临床推广。     

临床新型抗凝药物的研究进展

抗凝药物是一类通过影响凝血过程的不同环节进而抑制血液凝固的药物。目前传统的抗凝药物主要包括维生素K拮抗剂(VKA)、普通肝素(UFH)/低分子肝素(LMWH)。但是上述药物的缺点和不良反应使其在临床应用上受到诸多限制。近年来,针对凝血过程中的关键因子成功研发了多种新型抗凝药物,其中以直接凝血酶抑制剂和Xa因子抑制剂的研发最具有代表性,且多项临床试验已经证实这些药物并不劣于传统抗凝药物。本文对这些新药的临床研究进展进行综述。     

Lupus erythematosus tumidus in Japan: a case report and a review of the literature

Abstract

We report here the case of a 48-year-old Japanese woman showing plaque-forming scattered indurative papules on her face, buttock and extremities. Histological examination revealed a large amount of interstitial mucin deposition, and negative direct immunofluorescence was observed. The provocative phototesting reproduced the skin lesion, and the patient was diagnosed with lupus erythematosus tumidus (LET). A review of ten LET cases previously reported in Japan revealed that all of these cases had clinicopathological features similar to those reported for European cases, although not all of the former fully satisfied the European criteria.     

粉末直接压片法制备诺氟沙星片的工艺研究

目的进行诺氟沙星片粉末直接压片法的工艺研究。方法采用正交实验筛选处方,以物料流动性、硬度、溶出度、脆碎度、片重差异限度范围为评价指标选择最佳处方并验证。通过稳定性试验对片子的外观、含量、溶出度、有关物质进行考察。结果所选处方物料流动性好,有效成分溶出快,各项指标符合规定,稳定性良好。结论本工艺简单、便捷,质量稳定,适合工业化生产。